Journal: Nanomaterials

Article Title: Nanostructured Modifications of Titanium Surfaces Improve Vascular Regenerative Properties of Exosomes Derived from Mesenchymal Stem Cells: Preliminary In Vitro Results

doi: 10.3390/nano11123452

Figure Lengend Snippet: Effects of exo-hMSCs on HUVECs in vitro. ( A ) Internalization of exo-hMSCs by HUVECs. Representative confocal microscope images of HUVECs exposed to BODIPY™ TR ceramide-labeled exosomes ( red ) isolated from hMSCs seeded on T-untreated, TNs-25, TNs-80, and TNs-140 surfaces, or to BODIPY™ TR ceramide-labeled PBS (control). At 4 h post treatment with exosomes, HUVECs were stained with phalloidin ( green ), while nuclei were counterstained with DAPI ( blue ). Scale bar 20 μm. ( B ) Wound healing migration assay of HUVECs treated with the different exo-hMSCs preparations. Representative images of HUVECs migration after treatment for 24 and 48 h with vehicle (PBS), or with exo-hMSCs recovered from T-untreated, TNs-25, TNs-80, and TNs-140 surfaces. Scale bar 100 μm. ( C ) Migration distance calculated at 24 and 48 h post exosomes incubation. Data are expressed as the means ± SD. Note: * p < 0.05 and ** p < 0.01 mark significant changes in migration distance of HUVECs treated with hMSCs-derived exosomes compared to cells incubated with vehicle for the same culture time. Gene expression profiles of the endothelial cell-specific markers ( D ) VEGFR2, ( E ) CD31, and ( F ) VWF measured by real-time PCR in HUVECs treated for 48 h with the different exo-hMSCs preparations. Data are expressed as means ± SD of the target gene versus reference gene (TFRC) ratio and represented by 2 ΔΔCt . Note: ** p < 0.01, and *** p < 0.001 mark significant changes in gene expression level compared to HUVECs incubated with exo-hMSCs isolated from untreated surfaces for the same culture time; # p < 0.05 indicates significant changes in gene expression level versus HUVECs incubated with exo-hMSCs isolated from TNs-80.

Article Snippet: Surface morphologies were investigated using a scanning electron microscope (SEM, FEI, Nova Nano 430, NY, USA).

Techniques: In Vitro, Microscopy, Labeling, Isolation, Control, Staining, Migration, Incubation, Derivative Assay, Gene Expression, Real-time Polymerase Chain Reaction

Journal: Bioactive Materials

Article Title: MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration

doi: 10.1016/j.bioactmat.2021.08.034

Figure Lengend Snippet: Characterization of miR-29b/AuNPs (miR/NP) nano-delivery system. (a) Electrophoretic retardation analysis of miR-29b binding ability to AuNPs. (b) TEM image of miR/NP. (c) Hydrodynamic size and d) Zeta potential of AuNPs and miR/NP. (e) Intracellular delivery of Cy3-labeled miR/NP in hMSCs observed by confocal microscopy. (f) Cytotoxicity assay of hMSCs treated with different formulations detected by CCK-8 assay. (g) Cy3-positive cells treated with Cy3-miR/NPs detected by flow cytometry. (h) Fluorescence images of hMSCs co-stained by Calcein AM and PI treated with different formulations after 24 h and 48 h. Scale bar: 100 μm. Data are mean ± s.d., n = 5 per group.

Article Snippet: The surface morphology of scaffolds was characterized using scanning electron microscopy (SEM, Nova Nano 430, FEI, Netherlands).

Techniques: Binding Assay, Zeta Potential Analyzer, Labeling, Confocal Microscopy, Cytotoxicity Assay, CCK-8 Assay, Flow Cytometry, Fluorescence, Staining

Journal: Bioactive Materials

Article Title: MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration

doi: 10.1016/j.bioactmat.2021.08.034

Figure Lengend Snippet: Structure and morphology of MAHSs crosslinked with different concentrations of GTA. a) Optical images of each MAHS observed by 3D rotational microscopy. b) 3D distribution of miR/NP in MAHSs observed and reconstructed by confocal microscopy. miR-29b were labeled by Cy3. c) SEM images of MAHS-1 with and without miR/NP loading.

Article Snippet: The surface morphology of scaffolds was characterized using scanning electron microscopy (SEM, Nova Nano 430, FEI, Netherlands).

Techniques: Microscopy, Confocal Microscopy, Labeling